Abstract: Our electroporation microchip precisely controls the electric field on cells without bubble generation in microchannel by utilizing ionic conductivity of a polyelectrolyte hydrogel. pDADMAC (poly diallyldimethylammonium chloride) delivers electric potential from the electrode reservoir to the cells in microchannels Bubble generation is completely blocked without a high voltage source or a pulse generator . According to the calculation with CFD-ACE, the electric field across the salt bridges is around 1.3 kV/cm which meets the general electropermeation field strength (0.3-1 kV/cm).

Abstract: An efficient 3D-asymmetric microelectrode system for high-throughput was designed and fabricated to enhance sorting sensitivities to the dielectric properties—size, morphology, conductivity, and permittivity—of living cells. The principle of the present system is based on the use of the relative strengths of negative dielectrophoretic and drag forces, as in a conventional 3D-microelectrode system. Whereas the typical 3D-microelectrode system has a constant electric field magnitude due to the constant width of the microelectrodes and a fixed gap between face-to-face microelectrodes,

Abstract: This paper presents an integrated cell processor for the automatic handling of individual embryo cells. The integrated processor can perform various functions such as cell transport, isolation, orientation, and immobilization. These functions are indispensable and frequently used for the manipulation of single cells, but can only be carried out by a skillful operator. The purpose of this study was the integration and automation of these functions for effective cell manipulation, using a MEMS approach. The isolation of a cell was performed using polypyrrole (PPy) valves in a microchannel into which cells were transported.

Abstract: In this paper, we present a novel integrated bio cell processor to handle individual embryo cells. Its functions are composed of transporting, isolation, orientation, and immobilization of cells. These functions are essential for biomanipulation of single cells, and have been typically carried out by a proficient operator. The purpose of this study is the automation of these functions for effective cell manipulation using a MEMS based bio cell processor. This device is realized with relatively simple design and fabrication process. To transport cells, microfluidic channel is employed. The isolation of a cell is performed by actuation of polypyrrole (PPy) valves.

Abstract: There is a great demand to manipulate biological cell autonomously since biologist should spend much time to obtain skillful manipulation techniques. For this purpose, we propose a cell chip to control, carry, fix and locate the cell. In this paper, we focus on the cell rotator to rotate individual biological cell based on a micro fluidics technology.